Aquatic GAMUT Eukaryote Metagenomics

Authors:

Erin Jones Zach Aanderud

Owners:

Erin Fleming Jones iUTAH Data Manager

Type:

Resource

Storage:

The size of this resource is 1.7 MB

Created:

Jul 31, 2017 at 6:10 p.m.

Last updated:

Jun 19, 2018 at 11:10 p.m.

Citation:

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Sharing Status:	Public
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Abstract

This dataset contains a .biom file of OTU (operational taxonomic units) classifications for eukaryotes at iUTAH GAMUT sites in three watersheds in May of 2015. Briefly, water column samples were filtered on 0.2 um membrane filters, which were dissolved and processed with MOBIO Power Soil DNA extraction kits. We sequenced 18S region amplicons using Illumina Hi-Seq and then a modified MOTHUR protocol (Silva v. 132 reference file, command codes in mothureukaryotes.txt) to generate OTU classifications. Sample metadata (including site information and sample chemistry from grab samples or sensor data) is in design.csv.

Subject Keywords

Coverage

Spatial

Coordinate System/Geographic Projection:

WGS 84 EPSG:4326

Coordinate Units:

Decimal degrees

Place/Area Name:

Multiple Watersheds

North Latitude

41.9615°

East Longitude

-110.8809°

South Latitude

40.4010°

West Longitude

-111.9795°

Temporal

Start Date:	05/08/2015
End Date:	05/12/2015

Streets Terrain Satelite

Spatial Extent

Content

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Additional Metadata

Name	Value
Observed Variables	Rank abundance
Variable Description	Relative abundance of bacterial community taxa in water column
Data Collection Method	Streamwater aseptically vacuum filtered in the field or lab using autoclaved Nalgene filter cups and 47 mm 0.2 um membrane filters, were stored on dry ice or liquid nitrogen until extracted with MoBIO PowerMax Soil DNA kits. PCR-amplified 16S rDNA samples were sequenced using BYU’s DNA Sequencing Center (454-Pyrosequencing or Hi-Seq).
Data Processing Method	Sequences have been processed using MOTHUR (Schloss et al. 2009).

Related Resources

The content of this resource is derived from

http://www.hydroshare.org/resource/48fc6871c51d436b83000a8d29ddb702

Credits

Funding Agencies

This resource was created using funding from the following sources:

Agency Name	Award Title	Award Number
National Science Foundation	iUTAH-innovative Urban Transitions and Aridregion Hydro-sustainability	1208732

How to Cite

Jones, E., Z. Aanderud (2018). Aquatic GAMUT Eukaryote Metagenomics, HydroShare, http://www.hydroshare.org/resource/a80e4fa33a644691a29ab80b8cfc1aae

This resource is shared under the Creative Commons Attribution CC BY.

http://creativecommons.org/licenses/by/4.0/

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